Excision repair and patch size in UV-irradiated bacteriophage T4.
نویسندگان
چکیده
We determined the average size of excision repair patches in repair of UV lesions in bacteriophage T4 by measuring the photolysis of bromodeoxyuridine incorporated during repair. The average patch was small, approximately four nucleotides long. In control experiments with the denV1 excision-deficient mutant, we encountered an artifact, a protein(s) which remained bound to phenol-extracted DNA and prevented nicking by the UV-specific endonucleases of Micrococcus luteus and bacteriophage T4.
منابع مشابه
Dark repair of ultraviolet-irradiated deoxyribonucleic acid by bacteriophage T4: purification and characterization of a dimer-specific phage-induced endonuclease.
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ورودعنوان ژورنال:
- Journal of virology
دوره 40 2 شماره
صفحات -
تاریخ انتشار 1981